il15ra polyclonal antibody  (Proteintech)

Journal: International Journal of Molecular Medicine

Article Title: Genes regulated by interferon-γ in human uterine microvascular endothelial cells

doi: 10.3892/ijmm.20.5.689

Figure Lengend Snippet: Figure 2. Representative Western blotting for IL15RA, IFI30, MX1, CCNA1, and NQO expression in the soluble protein fraction extracted from myometrial (Myo) and endometrial (End) samples in the proliferative phase (Pro) and secretory phase (Sec). Protein extracts from placental tissue were used as a positive control.

Article Snippet: Goat antihuman interleukin-15 receptor α chain (IL15RA) polyclonal antibody (AF247) and recombinant human IL-15 receptor α chain/Fc chimera (147-IR, extracellular domain of IL-15 receptor α chain) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Western Blot, Expressing, Positive Control

Journal: International Journal of Molecular Medicine

Article Title: Genes regulated by interferon-γ in human uterine microvascular endothelial cells

doi: 10.3892/ijmm.20.5.689

Figure Lengend Snippet: Figure 3. Representative immunohistochemistry for IL15RA (A, C), IFI30 (D, E), MX1 (F, G), and CCNA1 (H). Scale bars, 50 μm. Immunoabsorption test (B, serial section to A) confirmed the specificity of the immunostaining. Basal layer of endometrium and adjacent myometrium (A, B, D and F). Functional layer of endometrium (C, E, G, and H). The arrows indicate the microvessels.

Article Snippet: Goat antihuman interleukin-15 receptor α chain (IL15RA) polyclonal antibody (AF247) and recombinant human IL-15 receptor α chain/Fc chimera (147-IR, extracellular domain of IL-15 receptor α chain) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Immunohistochemistry, Immunostaining, Functional Assay

Journal: EBioMedicine

Article Title: AI-guided discovery of the invariant host response to viral pandemics.

doi: 10.1016/j.ebiom.2021.103390

Figure Lengend Snippet: Fig. 6. ViP signatures reveal an interplay between IL15-storm and NK cell dysfunction in fatal COVID-19. (a) Bubble plots of ROC-AUC values (radius of circles are based on the ROC- AUC) demonstrating the direction of gene regulation (Up, red; Down, blue) for the classification based on the 20 gene severe ViP signature (top) and 166 gene ViP signature (bottom) in following datasets. RNASeq data (GSE115203) from PBMCs and sorted NK cells from PBMCs incubated with uninfected A549 cells for 12 hrs compared to infected A549 cells. PBMCs treated with IL15 compared to IL2 (GSE77601). RNASeq analysis of NK cells (GSE89484) treated with GSK-J4 compared to DMSO. Skin tissue in mice (GSE45551) is treated with anti-IL15RB antibody compared to PBS. (b) RNASeq data of NK cells isolated from two donors prior to vaccination compared (left) to days 1, 3, and 7 post-TIV vaccination like panel A. RNASeq data of NK enriched and NK depleted PBMCs from healthy donors compared to 30 day post-vaccination like panel A. (c, d) Heatmap of 20-gene (panel c) and 166- gene (panel d) ViP signatures in tissues collected during rapid autopsies on patients who succumbed to COVID-19. Genes are ranked according to the strength of differential expres- sion (T-test) in lung tissue between normal and infected tissue. (e) Box plots of IL15 and IL15RA in samples from varying severity of COVID-19. (f-h) Violin plots show levels of plasma IL15 in COVID-19 patients stratified by disease acquity (F), by clinical severity (G) and by gender and age (H). Welch’s two sample unpaired t-test is performed to compute the p values. See also Table S5 for patient metadata.

Article Snippet: Tissues were then incubated with rabbit IL15RA polyclonal antibody (1:200 dilution; proteintech , Rosemont, IL, USA; catalog# 16,744 1-AP) for 1.5 h and mouse IL15 monoclonal antibody (1:10 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA; catalog# sc-8437) at room temperature in a humidified chamber and then rinsed with TBS or PBS 3x, 5 min each.

Techniques: Incubation, Infection, Isolation, Clinical Proteomics

Journal: EBioMedicine

Article Title: AI-guided discovery of the invariant host response to viral pandemics.

doi: 10.1016/j.ebiom.2021.103390

Figure Lengend Snippet: Fig. 7. Lung alveolar cells contribute to the IL15 storm in fatal COVID-19. (a) Normal lung tissue obtained during surgical resection (left) or lung tissue obtained during autopsy stud- ies on COVID-19 patients (right) were stained for IL15 and IL15RA. Representative images are shown. Mag = 10X. (b) Violin plots display the intensity of staining for IL15RA (top) and IL15 (bottom), as determined by IHC profiler. (c) Hospital-free days analysis (45 days followup) of COVID-19 patients (GSE157103) limited to males less than 70 years old using the abundance of IL15 transcripts (intermediate and high groups) is displayed as Kaplan-Meier estimates (left) of cumulative probability of discharge and its relationship with days in hospital. (d) Cox-proportional hazard univariate analysis (right; top) of sViP (high vs low) is compared to ViP signature, Interferon Stimulated Gene-signatures (ISG1, PMID:15619625; ISG2, PMID:21478870), age, gender, ICU admission (icu) and mechanical ventilation (mv). Multivariate Cox-proportional hazard analysis (right; bottom) compares the variables that are significant in univariate settings, i.e., sViP, ICU admission (icu) and mechanical ventilation (mv). (e) Top: Schematic displays the workflow for patient blood col- lection and assessment of IL15 levels by mesoscale. Bottom: Bar (top) and violin (bottom) plots for the levels of IL15 cytokine (score = Z score of the log reduced mesoscale concentra- tion data). ROC AUC numbers indicate the strength of classification between patients with critical/fatal disease course vs. those with non-critical infection. (f) Summary of IL15 signaling and the hypothetical role of NK cells in the severity of COVID-19 infections.

Article Snippet: Tissues were then incubated with rabbit IL15RA polyclonal antibody (1:200 dilution; proteintech , Rosemont, IL, USA; catalog# 16,744 1-AP) for 1.5 h and mouse IL15 monoclonal antibody (1:10 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA; catalog# sc-8437) at room temperature in a humidified chamber and then rinsed with TBS or PBS 3x, 5 min each.

Techniques: Staining, Infection

Journal: EBioMedicine

Article Title: AI-guided discovery of the invariant host response to viral pandemics.

doi: 10.1016/j.ebiom.2021.103390

Figure Lengend Snippet: Fig. 8. Validation of ViP signature-guided therapeutic goals. (a-c) The 166-gene ViP signature-was used to classify liver biopsies from HCV-infected patients treated or not with directly acting anti-viral agents. ROC-AUC values are shown below each bar plot unless otherwise stated. (d) 166-gene ViP signature-based classification of blood samples from HIV- infected patients treated with anti-retroviral therapy (ART). (e) The compound EIDD-2801 (MK-4482; 500 mg/kg) or vehicle (Veh) was administered at indicated doses to Golden Syrian hamsters 4 h prior to intranasal infection with SARS-CoV-2. Hamsters were sacrificed on day 5 and lungs we analyzed by RNA sequencing. (f) Bar (top) and violin (bottom) plots using the ViP (left) or sViP (right) signature-based classification of lung samples from hamsters in E and uninfected controls. (g) Schematic showing the experimental design for validating the ViP signatures as useful tools to assess therapeutic efficacy. Uninf, uninfected; Den3 and Anti-CoV-2 indicate SARS-CoV-2 challenged groups that received either a control mAb or the clone CC12.2 of anti-CoV-2 IgG, respectively. (h) Bar (top) and violin (bottom) plots display the 166- and 20-gene ViP signatures in the uninfected and the SARS- CoV-2 challenged groups, treated with control or anti-CoV-2 IgG. (i-k) Lungs harvested from the 3 groups of hamsters were analyzed by H&E and IHC. Representative images are shown in I. Mag = 10X. Bar graphs in J display the abundance of cellularity and infiltrates in the lungs of the 3 groups, as determined by ImageJ. Violin plots in K display the intensity of staining for IL15RA (top) and IL15 (bottom), as determined by IHC profiler.

Article Snippet: Tissues were then incubated with rabbit IL15RA polyclonal antibody (1:200 dilution; proteintech , Rosemont, IL, USA; catalog# 16,744 1-AP) for 1.5 h and mouse IL15 monoclonal antibody (1:10 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA; catalog# sc-8437) at room temperature in a humidified chamber and then rinsed with TBS or PBS 3x, 5 min each.

Techniques: Biomarker Discovery, Infection, Retroviral, RNA Sequencing, Control, Staining